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KMID : 1005220120270010045
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Journal of Embryo Transfer
2012 Volume.27 No. 1 p.45 ~ p.50
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Effect of TES Extender on Sperm Characteristics and Viability of Frozen Semen in Miniature Pig
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Kim Sang-Hwan
Kang Hyun-Ah
Lee Myeong-Seop
Seo Kang-Suk
Yoon Jong-Taek
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Abstract
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The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition (37℃ for 20 sec, 45 sec and 75℃ for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : 60.3±2.4, NAI : 58.6±2.2%), TLE (61.3±2.4, 62.2±2.2%) extender significantly(p<0.05) increased than that in LEY (50.2±2.4, 54.5±2.2%) extender thawed at 75℃ for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE (66.1±3.2, 66.2±1.0%) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE (43.3±0.5%) while that in LEY (63.5±2.3%) is the highest. Therefore, these results suggest that TLE extender method for free- zing-thawing of miniature pig sperm increased the viability after thawing.
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KEYWORD
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miniature pig , glycerol , extender , sperm viability , DNA fragmentation
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